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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
BPS-60522 | 500 rxns | - | - |
3 - 15 business days* |
832.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The Glucocorticoid Receptor Pathway Reporter Kit is designed for monitoring the activity of the... more
Product information "GAL4 Reporter Kit (Glucocorticoid Receptor Pathway)"
The Glucocorticoid Receptor Pathway Reporter Kit is designed for monitoring the activity of the glucocorticoid signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low crossreactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.
Keywords: | Glucocorticoid Receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GAL4, GAL4/UAS, |
Supplier: | BPS Bioscience |
Supplier-Nr: | 60522 |
Properties
Application: | Monitor glucocorticoid signaling pathway activity, screening |
Species reactivity: | human |
Database Information
KEGG ID : | K05771 | Matching products |
UniProt ID : | P04150 | Matching products |
Gene ID | GeneID 2908 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | -20°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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