Cell-based Assays

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of cell viability, cytotoxicity and biological activity. By realistically representing the situation in vivo, these assays enable direct analysis of cell responses and play an important role in understanding disease mechanisms.

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of... read more »
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Cell-based Assays

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of cell viability, cytotoxicity and biological activity. By realistically representing the situation in vivo, these assays enable direct analysis of cell responses and play an important role in understanding disease mechanisms.

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MTT Cell Proliferation Assay Kit
MTT Cell Proliferation Assay Kit

Item number: Cay10009365-480

Cayman's MTT Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells through the plasma...
Application: Cell based assays
122.00€ *
Review
Protein Synthesis Assay Kit
Protein Synthesis Assay Kit

Item number: Cay601100-96

Cayman's Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating polypeptide chains, thereby...
Application: FC, fluorescence
543.00€ *
Review
AR (human) Reporter Assay Kit
AR (human) Reporter Assay Kit

Item number: IB-IB03001

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's reporter cells include the luciferase...
Keywords: AR, DHTR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Application: Functional activity screening
Species reactivity: human
From 1,448.00€ *
Review
One-Step Luciferase Assay System
One-Step Luciferase Assay System

Item number: BPS-60690-1

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component...
Keywords: Luciferin, Firefly luciferase, luciferase buffer,
Application: Firefly luciferase assays, HTS
From 238.00€ *
Review
Cell Meter(TM) TUNEL Apoptosis Assay Kit
Cell Meter(TM) TUNEL Apoptosis Assay Kit

Item number: ABD-22844

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that...
Application: TUNEL apoptosis assay
418.00€ *
Review
RORgamma (human) Reporter Assay Kit
RORgamma (human) Reporter Assay Kit

Item number: IB-IB04001

This human RAR-related orphan receptor gamma (RORgamma) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORgamma. Variant 2 mRNA comprises an alternate exon 1 that...
Keywords: RORC, NR1F3, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, RAR-related orphan receptor C, Retinoid-related...
Application: Functional activity screening
Species reactivity: human
From 1,448.00€ *
Review
Oxygen Consumption Rate Assay Kit
Oxygen Consumption Rate Assay Kit

Item number: Cay600800-96

The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen consumption is...
Keywords: OCR
Application: Fluorescence
542.00€ *
Review
CF(R)594 TUNEL Assay Apoptosis Detection Kit
CF(R)594 TUNEL Assay Apoptosis Detection Kit

Item number: BOT-30064

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3'- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF(R) dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to Biotium's exceptionally bright and photostable CF(R) fluorescent dyes, for...
810.00€ *
Review
FLICA(TM) 660 Caspase 3/7 Assay Kit, far-red fluorescence  (25-50 tests)
FLICA(TM) 660 Caspase 3/7 Assay Kit, far-red fluorescence...

Item number: ICT-9125

The far-red FLICA® 660-DEVD-FMK caspase-3 detection probe is comprised of the preferred affinity peptide sequence (DEVD) targeted by activated caspase-3 and caspase-7, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase-3 inhibitor probe forms an...
Keywords: Apoptosis kit, CASP3, CPP32, SCA-1, CPP-32, CASP-3, Apopain, Caspase-3, EC=3.4.22.56, Protein Yama, Cysteine protease...
Application: FM, FC, Apoptosis detection
367.00€ *
Review
FAM-FLICA(R) Caspases 3 & 7 Assay Kit
FAM-FLICA(R) Caspases 3 & 7 Assay Kit

Item number: ICT-93

This kits makes use of a cell-permeant green fluorecent probe (FAM-DEVD-FMK ) that binds to active caspase-3 & 7 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.
Keywords: Apoptosis kit
Application: FM, FC, Apoptosis detection
310.00€ *
Review
Cell Meter(TM) JC-10 Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays
Cell Meter(TM) JC-10 Mitochondrial Membrane Potential...

Item number: ABD-22801

Although JC-1 is widely used in many labs, its poor water solubility causes extraordinary inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, JC-10 has much better...
Application: FC, Apoptosis studies
418.00€ *
Review
Cell Meter(TM) Caspase 3/7 Activity Apoptosis Assay Kit * Blue Fluorescence*
Cell Meter(TM) Caspase 3/7 Activity Apoptosis Assay Kit *...

Item number: ABD-22795

This kit uses Ac-DEVD-AMC as a fluorogenic indicator for caspase-3 activity. Sufficient reagents to perform 500 assays. Using 25µl of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2,000 assays.
Application: Apoptosis assay
279.00€ *
Review
2 from 59 pages