Anti-Phosphotyrosine, mixed clones, clone 3G244

Protein tyrosine phosphorylation plays a central role in the transmission of signals from the cell surface to the nucleus.(3,7) Reversible phosphorylation of cellular proteins on specific tyrosine residues is involved in a variety of fundamental cellular processes, including survival, proliferation, differentiation, and transformation.(3,6,7) The use of antibodies which specifically recognize phosphotyrosine is widely accepted as one of the most powerful tools for detection, characterization, and purification of proteins that are either constitutively or inducibly phosphorylated on tyrosine (where even a single phosphorylated tyrosine residue is sufficient for detection).(1,2,4,5) , Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.