Anti-Phosphoserine, clone

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing , extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for , the assembly of protein complexes or may alter the 3-dimensional protein structure thus , modulating enzymatic activity or the ability to undergo protein-protein-interactions. , Modification of proteins on serine residues is mediated by serine/threonine kinases. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells, Western Blot: 1ug/ml with HRP/ECL detection. BSA/Tween 20 based blocking buffer. Do not use milk/casein for blocking and dilution! , Optimal dilution to be determined by the researcher. Positive Control Included: Yes , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ,