Anti-phospho-Structural Maintenance of Chromosomes 1 Like 1 (Ser957) (SMC1, SMC1L1), clone 4i7

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). In response to ionizing radiation (IR), as part of the intra-S-phase DNA damage checkpoint, ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 (3). In cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment, SMC1 phosphorylation is ATM-independent (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra- S-phase checkpoint, the precise mechanismis not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.