Anti-phospho-SMAD4 (Thr277) (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP

Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor. Applications: Suitable for use in FLISA, Dot Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: FLISA: 1:1,000 , Dot Blot: 1:500, Immunohistochemistry: paraffin, Immunofluorescence: 1:10-1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap., , Note: Applications are based on unconjugated antibody.