Anti-phospho-SAPK, JNK (Thr183) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

Stress-activated protein (SAP) kinases are proline-directed, serine/threonine-specific kinases that require phosphorylation on tyrosine for phosphotransferase activity.SAP Kinase was originally shown to phosphorylate microtubule-associated protein-2, but c-Jun appears to be an important physiological target of this kinase. Consequently SAPK has also been called Jun Kinase. Phosphorylation of Ser-63 and Ser-73 of c-Jun by SAP kinases promotes activation of the transcription factor AP-1. cDNA cloning studies with rat and human libraries have shown that there are several isoforms of the SAP Kinase family. Many stimuli activate SAP Kinase, including heat shock, 7 hyperosmotic shock, UV irradiation, exposure to protein synthesis inhibitors such as cycloheximide and anisomycin, and treatment with proinflammatory cytokines such as tumor necrosis factor-a. Applications: Suitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1:500-1:1000 , ELISA: 1:10,000, Immunohistochemistry:1:50-1:100 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.