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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
R2031-29.100 | 100 µl | - | - |
3 - 19 business days* |
704.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
To effectively promote growth and cell division in a sustained manner, growth factors and... more
Product information "Anti-phospho-Ribosomal Protein S6 (Ser235/236), clone 5i128"
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3).The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a 19 amino acid region in the S6 carboxy-terminus (4,5). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25, Western Blot: 1:1000, Immunofluorescence: 1:25., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: | United States Biological |
Supplier-Nr: | R2031-29 |
Properties
Application: | FC, IF, WB |
Antibody Type: | Monoclonal |
Clone: | 5i128(2F9) |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, monkey, mouse, rat |
Format: | Purified |
Database Information
Handling & Safety
Storage: | -20°C (frozen cool packs) |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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