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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
P9544-15.50 | 50 µl | - | - |
3 - 19 business days* |
688.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The pyruvate dehydrogenase complex (PDC) is a nuclear-encoded mitochondrial matrix multienzyme... more
Product information "Anti-phospho-Pyruvate Dehydrogenase E1-alpha Subunit, (Ser293) (Pyruvate Dehydrogenase E1 alpha, Pyr"
The pyruvate dehydrogenase complex (PDC) is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293 which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites., , Applications: , Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 detects a band at ~43kD., Immunofluorescence (IC): 1:50-1:250, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | EC=1.2.4.1, Anti-phospho-PDHE1-A type I, Anti-phospho-Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
Supplier: | United States Biological |
Supplier-Nr: | P9544-15 |
Properties
Application: | ICC, IF, WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, mouse, rat |
Immunogen: | Synthetic peptide corresponding to surrounding the phosphorylated serine 293 of the human PDHE1 alpha protein. [Swiss-Prot #P08559] |
Format: | Purified |
Database Information
KEGG ID : | K00161 | Matching products |
UniProt ID : | P08559 | Matching products |
Gene ID | GeneID 5160 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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