Anti-phospho-Protein Kinase R (Thr451) (PKR, ds RNA-dependent Protein Kinase)

Double-stranded RNA-dependent protein kinase (PKR) is a 68kD protein that is induced by interferon and double-stranded RNAs (dsRNA) produced in virus-infected cells. Two dsRNA- binding domains in the N-terminus interact with dsRNA to modify the conformation of PKR and allow it to undergo autophosphorylation and activation. Once activated, PKR phosphorylates eIF2alpha, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction. The phosphorylation of two sites in the activation loop of the kinase domain, threonines 446 and 451, is critical for high level catalytic activity. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells stimulated with interferon alpha/beta (IFNa/b) and calyculin. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.