Anti-phospho-Myosin, light chain 2 (Ser19) (MLC2)

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2, and Ser9 by PKC and cdc2, is also reported, which inhibits myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Lack of phosphorylation sites on cardiac muscle isoform shows morphological and functional abnormalities on transgenic mice (6). Applications: Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunofluorescence/Immunocytochemistry: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.