Anti-phospho-MEK1 (Thr292) (Map Kinase Kinase, MAPK, Erk Kinase)

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 detected phosphorylated MEK1 (Thr292) in RIPA lysates of untreated A431 cells., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. , Quality Control Testing: Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated MEK1 (Thr292) in RIPA lysates of untreated A431 cells. Lysates from A431 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- MEK1 (Thr292) for 1 hours at room temperature. Lane 2 was preincubated with lambda phosphatase prior to primary antibody incubation. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-MEK1.