Anti-phospho-MARCKS (Ser152/156) (Myristolated Alanine Rich C Kinase Substrate)

Myristoylated alanine-rich protein kinase C substrate (MARCKS) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis (1). PKC phosphorylates serines 152, 156 and 163 of MARCKS (2), which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity (3,4). In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm (5). Recommended Dilution: Western Blotting: 1:1000 , Optimal dilutions to be determined by the researcher., , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.