Anti-phospho-MAP Kinase p44/42, (Tyr204) (MAPK1/MAPK3, Mitogen-activated Protein Kinase 1, MAP Kin

Human p44/42 MAP Kinase belongs to the family of signal transduction intermediates leading to cell proliferation or differentiation, known as MAPKs (mitogen-activated protein kinases). It plays an important role in the signal transduction pathways that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals.Activation of the protein requires phosphorylation of both tyrosine and threonine residues by upstream dual-specific kinases. Active MAPKs are responsible for the phosphorylation of a variety of effector proteins, including several transcription factors. Applications: Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000, Immunohistochemistry (paraffin): 1:50-1:100, Immunofluorescence/Immunocytochemistry: 1:100-1:200, Optimal dilutions to be determined by the researcher. Positive Control: NIH-3T3 cells, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.