Anti-phospho-MAP Kinase, p38 (Thr180, Tyr182) (MAPK, Erk2)

The p38 (CSBP, RK, or Mpk2) protein is the newest member of the MAP kinase family to be isolated. This protein was originally identified as a kinase phosphorylated on tyrosine in response to treatment of cells with LPS2. Subsequently, p38 (CSBP1/CSBP2) was identified as the target of pyridinyl-imidazole compounds, which inhibit the production of interleukin-1 and tumor necrosis factor from stimulated human monocytes3. p38 and JNK appear to be activated by many of the same stimuli, nevertheless, differences in the extent and timing of activation of p38 and JNK have been observed. p38 and JNK are activated by distinct MAPK kinases: MKK4 activates both JNK and p38, whereas MKK3 and MKK6 activate p38 specifically. Studies have implicated p38 in the phosphorylation of the small heat shock proteins Hsp27, MAPKAP kinase -, 2 and -3, and the transcription factors ATF2, Elk-1 and CHOP7. In addition, p38 likely mediates increased cytokine expression and some aspects of programmed cell death. Phosphorylation of p38 MAPK associated with the activation of NFkB may be related to human crescentic glomerulanephritis. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, Immunohistochemistry: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.