Anti-phospho-MAP Kinase, (Erk 1,2, phospho MAP Kinase, Extracellular Signal Regulated Kinase) (Biot

Applications: , Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.05ug/ml., Western Blot: 0.5ug/ml (ECL) Recommended blocking buffer: Casein/Tween-20., Immunocytochemistry: 1-10ug/ml, Immunoprecipitation: 1ug/10e6 cells (Pervanadate treated) A431 orHepG2 cells), Optimal dilutions to be determined by the researcher. Positive control Included: H2030-03H1 HepG2 Cell Lysate, Pervanadate Treated. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.