Anti-phospho-JNK (Thr183, Tyr185) (Stress-Activated Protein Kinase, SAPK, Jun-terminal Kinase), clon

The stress-activated protein kinase/Junterminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1-4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4-7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4-7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors. Application: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000. Incubate membrane with diluted S0096-04C in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:250, Flow Cytometry: 1:400, Immunofluorescence (IF-IC): 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.