Anti-phospho-Integrin, beta3 (Tyr773) (Platelet Glycoprotein IIIa, CD61, GP3A, GPIIIa, ITGB3, NAIT,

Integrin b3, also known as CD61, is a 130kD transmembrane glycoprotein that binds noncovalently in complexes with integrin a subunits (aIIb, av) to form the functional receptor that binds to specific extracellular matrix proteins (e.g., fibronectin, vitronectin, etc.). Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic ,development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrin receptors on the cell surface and their binding to the extracellular matrix leads to the formation of focal contacts and the activation of various signal transduction pathways. Phosphorylation of tyrosine 773 on Integrin b3 activates the receptor and promotes cell adhesion. Tyr773 is commonly referred to as Tyr747, the corresponding site in the chicken Integrin b3 protein. Applications: Suitable for use in Western Blot adn Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1:500-1:1,000, Immunohistochemistry:1:50-1:100 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.