Anti-phospho-Insulin Receptor (Tyr1150/1151) (IR), clone

The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain and a transmembrane and intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assenbly of signal transduction complexes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.1ug/ml, Western Blot: 0.5ug/ml for HRPO/ECL detection., Blocking Buffer: Casein/Tween20, Optimal dilutions to be determined by the researcher. Positive Control Included: I7661-22T1: Insulin Receptor, phosphorylated (Tyr1150/1151) (IR), Positive Control (HEK-293 cells treated with insulin), Western Blot: Add 200ul 2x SDS-PAGE sample buffer and incubate at 90°C for 5 for HRPO/ECL detection. 20ul/lane (mini gel). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.