Anti-phospho-Histone H3, (Ser28)Ubiquitin

Anti-phospho-Histone H3, (Ser28)Ubiquitin
Item number Size Datasheet Manual SDS Delivery time Quantity Price
H5110-12M4.20 20 µl - -

3 - 19 business days*

H5110-12M4.100 100 µl - -

3 - 19 business days*

Modulation of chromatin structure plays an important role in the regulation of transcription in... more
Product information "Anti-phospho-Histone H3, (Ser28)Ubiquitin"
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Flow cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 , Immunoprecipitation: 1:25 , Immunofluorescence (IF-IC): 1:400 , Immunofluorescence (frozen): 1:800, Flow Cytometry: 1:400 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: H5110-12M4


Application: FC, IF, IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Drosophila, Hamster, Human, Mouse
Immunogen: Synthetic peptide (KLH-coupled) corresponding to the amino terminus of histone H3 phosphorylated on Ser28.
Purity: Purified by Protein A affinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: -20°C)
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow. more
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Anti-phospho-Histone H3, (Ser28)Ubiquitin"
Write a review
or to review a product.