Anti-phospho-Histone H3 (Ser28), clone 4H101

The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms. Metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines, Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml ~18kD band is detected only in treated cell lysate, Optimal dilution determined by the researcher. Positive Control: Anti-Fas mAb treated Jurkat cell lysates. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.