Anti-phospho-Histone H3 (Ser10) (Mitosis Marker)

Applications:, Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml detected histone H3 on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system., Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate., Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells., Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-47H: IgG, H&L (HRP) Pab GtxRt, I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt, I1904-47T: IgG, H&L (HRP) Pab GtxRt, I1904-50T1: IgG, H&L (HRP) Pab GtxRt, I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt, I1904-40D: IgG, H&L (FITC) Pab Gt xRb, I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb , I1904-46N: IgG, H&L (FITC) Pab Gt xRb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after recei. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.