Anti-phospho-Histone H2A.X (Ser139), Biotin conjugated, clone 2T14

Applications: , Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml detects phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine. Untreated, and staurosporine-treated Jurkat cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-08B (0.5ug/ml). Proteins were visualized using a streptavidin, horseradish peroxidase conjugate and a chemiluminescence detection system. , Optimal dilutions to be determined by the researcher. Positive Control: Staurosporine-treated Jurkat cell lysates. Recommended Secondary Reagents: S7973-97A: Streptavidin (HRP), Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.