Anti-phospho-Estrogen Receptor alpha (Ser118) (ERa), clone 4H323

Applications: , Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 detects phospho-estrogen receptor (Ser118) in RIPA lysates from MCF-7 cells treated with estradiol and EGF, and recombinant estrogen receptor phosphorylated in vitro with MAP Kinase 2/Erk2, active. Lysates from estradiol and EGF treated MCF-7 cells (lanes 1 and 2) or recombinant estrogen receptor protein phosphorylated with MAPK2/Erk2 (lanes 3 and 4) were resolved by electrophoresis and transferred to nitrocellulose. Following blocking of the membrane, the nitrocellulose was either treated with lambda phosphatase (lanes 1 and 3) or left untreated (lanes 2 and 4), then probed with anti-phospho- Estrogen Receptor (Ser118) (1:2000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.