Anti-phospho-Estrogen Receptor alpha (Ser118) (ERa), clone 3A15

Estrogen receptor alpha (ER alpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER alpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ER alpha activity (3,4). ER alpha is phosphorylated on multiple sites (5). Serines 104, 106, 118 and 167 are located in the N-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER alpha activity. Ser118 may be the substrate of the transcription regulatory kinase cdk7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4). Recommended Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50, Optimal dilutions to be determined by the researcher. , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.