Anti-phospho-CREB1, (Ser133) (Cyclic AMP-responsive Element-binding Protein 1, CREB-1, cAMP-respons

CREB1 is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in response to extracellular signals and are essential regulators of adaptive behavior and long-term memory formation. Activation of CREB1 by protein kinase A-mediated phosphorylation has been implicated in the survival of mammalian cells. Mice lacking CREB in the central nervous system during development show extensive apoptosis of postmitotic neurons. CREB binds as homo- and heterodimers to promoters containing CRE and activator protein 1 (AP-1) sites to alter target-gene expression). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000, Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.