Anti-phospho-c-Fos (Ser374) (Fos), clone

The immediate early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the c-Fos protein and primes c-Fos for additional phosphorylation at Thr325. During transient MAPK activation, activity declines before accumulation of the c-Fos protein. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Recommended Dilution: ELISA: 0.1ug/ml, Immunohistochemistry: 1:50, Western Blot: 0.5ug/ml (ECL). Recommended blocking buffer: CPPT [0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) PVP, 0.1% (v/v) Tween 20], 2x PBS, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.