Anti-phospho-Bcl XL, phosphorlayted (Thr115)

Bcl-XL (28kD) is part of the Bcl-2 family, which are important regulators of apoptosis. Bcl-X has two isoforms, Bcl-XL (long) a 241 amino acid protein and shares signi?cant homology with Bcl-2. Bcl-XS (short) a 178 amino acid protein lacking a 63 amino acid domain near the transmembrane region that is well conserved among members of the Bcl-2 family. Like Bcl-2, Bcl-XL has the ability to inhibit apoptosis and is widely studied for its role in tumor generation. In a recent study, analysis of Hodgkin's disease showed that 71% of cases (114/160) showed immunohistochemical reactivity with Bcl-XL in Reed-Sternberg cells. In some cases, Bcl-XL reactivity was present in Reed-Sternberg cells that were negative for CD15 and CD30. Atypical hyperplasias (0/28) were negative for Bcl-XL. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 for detection of coated peptide (100ng/well), Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.