Anti-phospho-Amyloid Precursor Protein (Thr743/668) (Amyloid beta A4 Protein, ABPP, APPI, APP, Alzhe

Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer's disease patients and Downs's Syndrome patients. The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA:1:4,000 , Western Blot:1:500-1:1,000, Immunohistochemistry:1:50-1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.