Anti-EGFR (Phospho-Tyr1068) (Clone RM443)

Epidermal growth factor receptor (EGFR), also known as HER1 and ErbB-1, is a cell surface receptor and member of the EGF family of receptor tyrosine kinases with roles in cell proliferation, differentiation, and survival. It is a transmembrane receptor composed of an intracellular tyrosine kinase domain, a transmembrane lipophilic segment, and an extracellular domain that is expressed in epithelial, mesenchymal, and neuronal tissues. Under unstimulated conditions, EGFR is an auto-inhibited monomer in the plasma membrane. Upon canonical ligand binding, EGFR undergoes homodimerization or heterodimerization with HER2, HER3, or HER4, which induces a conformational change in the cytoplasmic domain that facilitates autophosphorylation and intracellular signaling. EGFR contains five C-terminal autophosphorylation sites, namely tyrosine 1068 (Tyr1068), Tyr1148, Tyr1173, Tyr1086, and Tyr992. EGFR (phospho-Tyr1068) is associated with prolonged progression-free survival in patients with non-small cell lung cancer (NSCLC). Cayman's EGFR (Phospho-Tyr1068) Rabbit Monoclonal Antibody can be used for Western blot (WB).Synonyms: Epidermal Growth Factor Receptor, ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, HER1. Immunogen: A phospho-peptide corresponding to human phospho-EGFR. Clone Designation: RM443. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: WB. Cross Reactivity: (+) EGFR (Phospho-Tyr1068), (-) EGFR without phosphorylation at Tyr1068.