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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
H5110-14R1.100 | 100 µl | - | - |
3 - 19 business days* |
1,060.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in... more
Product information "Anti-dimethyl-Histone H3 (Lys79), clone"
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Applications: Suitable for use in Western Blot, Dot Blot and Histone-Peptide assay. Chromatin Immunoprecipitation (ChIP) has been reported by an independent laboratory. Other applications not tested. Recommended Dilution: Western Blot: 1:4000 Recombinant Histone H3 or acid extracted HeLa cell proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14R1. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system., Peptide Dot Blot Analysis: 1:2500 dilution of this lot detected the immunizing peptide containing dimethyl-lysine 79. Some crossreactivity with peptide containing monomethyl-lysine 79 was also seen. No crossreactivity to other related histone peptides was found. The antibody did not recognize a histone peptide containing the dual modification of dimethyl-lysine 79 and phospho-threonine 80., Histone-Peptide Specificity Analysis: 1:25-1:1600 dilution of this lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex(R) microspheres. Peptide containing dimethyl-lysine 79 was readily detected. Some crossreactivity with peptide containing monomethyl-lysine 79 was seen. The antibody did not detect a histone H3 peptide containing the dual modification of dimethyl-lysine 79 and phosphothreonine 80., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | H5110-14R1 |
Properties
Application: | Dot Blot, IP, WB, ChIP |
Antibody Type: | Monoclonal |
Clone: | 5H66 |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human |
Format: | Affinity Purified |
Database Information
Handling & Safety
Storage: | -20°C (frozen cool packs) |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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