Anti-acetyl-Lysine

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 dilution detected:, Acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones., GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF., Immunoprecipitation: 5ul immunoprecipitated in vitro acetylated recombinant p300., Optimal dilutions to be determined by the researcher. Positive Control: TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.