Anti-acetyl-Histone H3 (Lys9)

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Applications: Suitable for use in Western Blot, ChIP and Beadlyte Histone-Peptide Specificity Assay. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells., Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9., Beadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex(R) microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed., Optimal dilutions to be determined by the researcher. Positive Control: TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.