Anti-acetyl-Histone H3 (Lys23)

Applications: , Suitable for use in Western Blot and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:100,000-1:200,000 dilution of this lot detected acetyl-histone H3 (Lys23) in acid extracts from sodium butyrate treated HeLa cells. The antibody does not detect unacetylated recombinant histone H3., Chromatin Immunoprecipitation (ChIP): 0.5ul of this lot preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H3 K23R substitution., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.