Anti-acetyl-Histone H3 (Lys14)

Applications: , Suitable for use in Western Blot, Beadlyte? Histone-Peptide Specificity Assay and Chromatin IP. Other applications not tested. Recommended Dilution: Western Blot Analysis: A 1:1000-1:5000 dilution detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells , Beadlyte? Histone-Peptide Specificity Assay: 1:1000-1:5000 were incubated with histone H3 peptides containing various modifications conjugated to Luminex? microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected., Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.