MAPK Family BioAssay Sample Kit, Affinity Purified

P44/42 MAPK (Erk1 and Erk2), SAPK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, and the differentiation and control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation. MAPK Family Antibody Sampler Kit contains 20ul of each primary antibody (p44/42 MAP Kinase, SAPK/JNK, p38 MAP Kinase) and 50ul of anti-rabbit IgG secondary antibody labeled with HRP. , Kit Components:, M2352-40A: p44/42 MAP Kinase Antibody 1x20ul, M2352-40B: SAPK/JNK Antibody 1x20ul, M2352-40C: p38 MAP Kinase Antibody 1x20ul, M2352-40D: Rabbit ant-Human IgG, HRP, 1x50ul, Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.