Intermediate Neurofilament Antibody Panel (NF-L, NF-M,NF-H)

Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits. It appears that the essence of this assembly process is the heterotetrameric unit, which exists in two forms: NFL-NFM and NFL-NFH. Tetramer pairs form protofilaments and eight protofilaments constitute the final 10 nm intermediate filament. NFL is essential for the correct assembly of neurofilaments and maintenance of axonal calibre and NFM not only forms cross-bridges, stabilizing the filament network, but also helps in longitudinal extension. NFH also forms cross-bridges and may interact with micro-tubules and other cytoskeletal elements. Neurofilament accumulations are found in several human neurological diseases. These include ALS, Lewy bodies in Parkinson's disease and some dementias (neurofilaments are a component of the Lewy body along with a-synuclein), Alzheimer's disease, progressive supranuclear palsy, Charcot-Marie-Tooth disease, diabetic neuropathy, and giant axonal neuropathy. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339. Sakaguchi, T et al. (1993) Neurosci Lett 153, 65-68. Ammar Al-Chalabi and Christopher C. J. Miller (2003) BioEssays 25, 346-355. Contains: ARG52348: Anti-Neurofilament NF-L antibody [DA2]: Mouse mAb: Hu, Ms, Rat: FACS, ICC/IF, IHC-P, IHC-Fr, WB: 50 µl and ARG52351: Anti-Neurofilament NF-M antibody: Chicken pAb: Ms, Rat: ICC/IF, IHC-Fr, WB: 50 µl and ARG52347: Anti-Neurofilament NF-H antibody: Chicken pAb: Cow, Ms, Rat: ICC/IF, IHC-Fr, WB: 50 µl and ARG65350: Goat Anti-Mouse IgG antibody (HRP): Goat pAb: Ms: ELISA, IHC-P, WB: 50 µl.