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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
W3037-10.100 | 100 µg | - | - |
3 - 19 business days* |
901.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature... more
Product information "Anti-Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)"
Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development. Applications: Suitable for use in Immunohistochemistry and Direct ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 10ug/ml detects Wnt-8b on mouse fetal brain tissue sections. Sections were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted W3037-10 overnight at 4°C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagent to detec human or mouse Wnt8b. The detection limit is ~5ng/well. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: | United States Biological |
Supplier-Nr: | W3037-10 |
Properties
Application: | ELISA, IHC |
Antibody Type: | Monoclonal |
Clone: | 7H100 |
Conjugate: | No |
Host: | Rat |
Species reactivity: | human, mouse |
Immunogen: | E. coli-derived recombinant mouse Wnt-8b |
Format: | Affinity Purified |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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