Anti-VAMP 1 (Vesicle Associated Membrane Protein 1, Vesicle-associated Membrane Protein 1, VAMP1, VA

VAMP-1 (vesicle-associated membrane protein 1, also synaptobrevin-1/SYB1) is an 18kD member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa1-96) and a 22aa transmembrane domain (aa97-118). There is one coiled-coil region between aa33-93. Multiple splice variants are known that show two, three, four and 81aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. over aa1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Immunohistochemistry: 25ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human VAMP-1 (aa1-96). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.