Anti-Ufc1, CT (UFC1, Ubiquitin-fold modifier-conjugating enzyme 1) (Azide free) (APC)

Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway . These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, FLISA, Recommended Dilution: FLISA: 1:1,000, Western Blot: 1:100-500, Immunohistochemistry: 1:10-50, Flow Cytometry: 1:10-50, Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap., , Note: Applications are based on unconjugated antibody.