Anti-Tumor Necrosis Factor Receptor 1, p55 (TNFR1, CD120a)

Tumor necrosis factor alpha (TNFalpha), also known as cachectin, is a 17.5kD, 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNFalpha is produced in both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor 1 (TNFR 1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNFR 2), expressed on endothelial and most immune cells. TNF signaling regulates hematopoiesis, differentation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune suveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune disease, diabetes, and atherosclerosis. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 using ECL, Immunoprecipitation: 12.5ug/ml, Immunohistochemistry (paraffin): 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.