Anti-Tumor Necrosis Factor alpha (TNFa, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2

Tumor Necrosis Factor alpha (TNFa) is a 17kD cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role. Applications: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1-10ug/ml, Flow Cytometry: 1:10-1:50, 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required., Immunohistochemistry: Frozen sections, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.