Anti-Tumor Necrosis Factor alpha (TNFa), clone 5E852

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin-fixed frozen tissues, Optimal dilutions to be determined by the researcher. Matched Pairs: Capture: T9160-32A, Detection: T9160-18A , Hybridoma: X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.