Anti-Tumor Necrosis Factor Alpha (TNF-alpha)

Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17kD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells. It causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative microorganisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of microorganisms and by various cytokines, but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intracellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor crosslinking. Applications: Suitable for use in ELISA and Neutralization. Other applications not tested. Recomended Dilutions: Optimal dilutions to be determined by the researcher. Affinity Constant: 5.7x10e-10M, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.