Anti-Tumor Necrosis Factor Alpha (TNF-alpha) (FITC)

TNF-alpha can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody, which recognizes soluble, membrane and receptor bound TNF-alpha. , , Applications:, Suitable for use in Flow Cytometry, Western Blot, and immuno assays. Other applications not tested. Recommended Dilutions: Functional Assays: 1:50, Western Blot: 1:50, Optimal ddilutions to be determined by the researcher. Storage and Stability: For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.