Anti-TRF2 (Telomeric Repeat-binding Factor 2, TTAGGG Repeat-binding Factor 2, Telomeric DNA-binding

TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length (1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (1). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3). Applications: Suitable for use in Western Blot, ChIP, ELISA, Flow Cytometry, Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 2ug/ml, Flow Cytometry: 0.1ug/10e6 cells, Immunohistochemistry (frozen): 5ug/ml, Immunoprecipitation: 2ug/10e6 cells, Western Blot: 2-4ug/ml, Immunofluorescence (IC): 1:10-1:500, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.