Anti-Transforming Growth Factor beta (TGFb), clone 3H282

ransforming growth factor beta (TGF-beta) has three isoforms (TGF-beta1, TGF-beta2, and TGF-beta3) with similar functions. The cytokine is a homodimer linked by disulfide bind. Inside cells, the cytokine forms a small latent complex with latent associated peptide (LAP). This small complex binds to latent TGF-beta binding protein (LTBP) to be secreted to extra-cellular matrix. Disassociation of the latent proteins from TGF-beta results in the release of the cytokine to its receptor. The process is called activation, which can be influenced by various factors, including proteases, metalloproteases, extreme pH, mild acidic condition, reactive oxygen species and integrins. , TGF-beta is an anti-proliferation factor in normal cells. It increases the synthesis of p15 and p21, which can block the cyclin: CDK complex, and causes cells to stop at G1 phase. The cytokine can induce apoptosis through both SMAD and DAXX pathways. In cancer cells, TGF-beta signaling is altered and TGF-beta no longer stops cell proliferation. , Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml allows detection of 5ng/lane TGF-beta using human platelets, Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.