Anti-TLR4 (Toll Like Receptor-4, MD2)

TLR4 (Toll-like receptor 4) is a mammalian homologue of the Drosophila Toll protein. The human TLR4 is a 799-residue type-1 transmembrane protein. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of the IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB. Applications: Suitable for use in Neutralizing, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:3000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.