Anti-TLR4 (Toll Like Receptor-4, MD2), FITC conjugated, clone 3F5

TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. , Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-83Y1: IgG2a Negative Control (FITC), , Hybridoma: , Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.