Anti-Tissue Factor

Tissue Factor (TF) is expressed in the plasma membranes of many cell types. It is an integral membrane glycoprotein consisting of a single chain molecule of 44kD. TF consists of an extracellular domain (residues 1-219), a transmembrane domain (residues 220-242) and the C-terminal intracellular domain (residues 243-263). Most abundant in the tissue adventitia, TF becomes exposed to blood at the site of vascular injury. The availability of TF is important in initiating coagulation by acting as a receptor for both the zymogen and protease forms of plasma Factor VII (FVII and FVIIa). It also mediates the conversion of bound FVII to FVIIa. The binding of FVII to TF in the presence of a negatively charged surface such as a phospholipid (or cell surface) promotes the auto activation of FVII by FVIIa. The TF-FVIIa complex in the presence of calcium ions proteolytically activates FIX and FX. These enzyme products are then capable of activating FVII to FVIIa by feedback amplification. The activity of TF-.VIIa activity is regulated by a TFPI (Tissue Factor Pathway Inhibitor), a member of the Kunin superfamily of protease inhibitors. TFPI contains three Kunitz domains and is able to bind and inhibit the TF-FVIIa complex in the presence of activated FX and calcium ions. Antithrombin has also been reported to inhibit FVIIa activity in the presence of TF and heparin. Although a membrane protein, low levels of TF products have been demonstrated in plasma. Application: Suitable for use ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.